Fig. 8. Double-label immunofluorescence of retinal cross-sections reveals the intricate relationships between Chx10, CycD1 and Kip1 proteins. (A-C) Chx10 and CycD1 are present in the same cells in wild-type retinas. (D-F) CycD1, like Chx10, is in a complementary set of cells relative to Kip1. (G-I) In Chx10-null retinas, CycD1 is largely able to maintain a complementary expression pattern with Kip1. (J-L) However, in CycD1-null retinas, Chx10 fails to maintain complementarity with Kip1. (M-N) High magnification confocal images of Chx10 (red) and Kip1 (green) staining show more clearly that Kip1 and Chx10 are present in the same RPCs in the CycD1-null retina, but not in the wild-type retina. Both images are within the neuroblast layer. Scale bars: in A, 50 µm for A-L; in M, 10 µm for M,N.