Fig. 5. Precocious differentiation of the retinal stem cells by inhibition of the Wnt canonical pathway with LEF1. (A-D) Transverse sections of stage 21 embryo electroporated with control (A,B) or LEF1 (C,D) stained with DAPI (A,C) and the co-electroporated GFP (B,D). Note that the cell clump in the dorsal retinal margin strongly expressed GFP (arrow). (E-H) The incorporation of BrdU (red) in the control (F) and LEF1-electroporated (H) retina. The co-electroporated GFP is shown in E,G. (F,H) Pseudo-colored DAPI signals are shown in green. (I-P) The expression of co-electroporated GFP (I,K,M,O), NF-M (J,L), Islet1 (N,P) in the cell clump of LEF1-electroporated retina adjacent to the lens (le). Arrowheads indicate the ectopic NF-M expression. Scale bars: 100 µm.