Fig. 2. lim-6 and ceh-14 are expressed in the PVT neuron and coordinately affect zig-4::gfp expression. (A) Embryonic expression of lim-6 and ceh-14 reporter gene constructs in PVT (see Materials and Methods for details on expression constructs). Postembryonic expression of lim-6 ceh-14 in PVT has been previously reported (Cassata et al., 2000; Hobert et al., 1999b). (B) Effect of lim-6 ceh-14 on zig-4::gfp (otIs20) expression. The inset shows an enlargement of the tail region with a gfp-fluorescing PVT cell. White star denotes gut autofluorescence. Note that other zig-4::gfp expressing cells are not affected by lim-6 ceh-14. See Fig. 3 for quantitative data on all PVT-expressed zig genes. White arrows points to PVT, which lacks a gfp signal in panel B.