Fig. 2. Expression pattern of PDF2. (A) RNA gel blot analysis of PDF2 mRNA. Total RNA was isolated from whole seedlings (Se), roots (R), leaves (L), stems (St), flower-bud clusters (F) and siliques (Si). Seedlings were grown for 10 days in MS plates. The EF1 probe was used as a control for loading and transfer. (B-G) In situ localization of PDF2 mRNA. Longitudinal sections of a vegetative shoot meristem of a 10-day-old seedling (B), apical inflorescence and floral meristems (C), a flower bud (D), ovule primordia (E), a quadrant-stage embryo (F) and an early globular-stage embryo (G) were hybridized with the antisense PDF2 RNA probe.