Fig. 3. Expression patterns of transcripts enriched in the wing/hinge sample. The expression patterns of the transcripts are shown in the overall rank order according to fold change with the most highly enriched shown first (Table 2). All genes are expressed in the columnar epithelium except where noted. (A) CG17278 is strongly expressed throughout the wing pouch, but not in cells at the DV margin, and expressed in a small cluster of cells in the dorsal hinge. (B) defective proventriculous (dve) is weakly expressed throughout the wing pouch, but not in cells at the DV margin. (C) POU domain protein 2 (pdm2) is expressed throughout the wing pouch. Expression levels vary with higher expression in cells probably corresponding to vein precursors. (D) CG8780 is expressed in cells of the mesopleura and ventral hinge. (E) ß-galactosidase/CG3132 (ß-gal) is expressed in a cluster of cells in the dorsal hinge and in blood cells that adhere to the anterior ventral margin of the disc. (F) Nep1/CG5894 is expressed in the mesopleura (arrowhead) and dorsal and ventral hinge. (G) odd paired (opa) is expressed in the mesopleura (arrowhead) and dorsal and ventral hinge. (H) CG15001 is weakly expressed throughout the wing pouch. (I) CG15489 is expressed weakly throughout the wing pouch. Expression levels vary with higher expression in cells probably corresponding to vein precursors. (J) Cyp310a1 (Cyp) is expressed throughout the wing pouch except in cells at the DV and AP margins. (K) CG15488 is expressed throughout the wing pouch. Expression levels vary with higher expression in cells probably corresponding to vein precursors. (L) CG15000 is weakly expressed throughout the wing pouch. (M) CG14534 is specifically expressed in cells that give rise to the posterior margin of the wing. (N) BG:DS00797.2/CG9008 (797.2) is strongly expressed throughout the wing pouch with some modulation in levels that does not appear to correlate with known features such as veins. Transcripts are also present at low levels in adeptihial cells (arrowhead, out of the plane of focus). (O) CG4861 is expressed very weakly throughout the wing pouch. (P) CG6469 is expressed in peripodial cells with higher levels of expression in ventral cells. (Q) CG14301 expression is weak but elevated expression was seen in cells at the anterior disc margin and in four clusters of cells in the wing pouch and overlying peripodial membrane. (R) Doc2/CG5187 is expressed in four patches of cells including a group of cells in the body wall (arrowhead). (S) UDP-glucosyl transferase 86Di/CG6658 (Ugt) is expressed in a diffuse pattern with elevated expression along the anterior disc margin, extending into the body wall region, and in intervein regions. (T) anachronism (ana) is expressed in clusters of cells and individual cells, probably neuroblasts. A cluster of cells in the body wall region express ana (arrowhead). (U) CG5758 is expressed in the hinge region. Expression is modulated so that the pattern has a patch-like appearance. (V) CG8381 is expressed strongly in the wing pouch with modulation at the DV margin and the presumptive veins. The gene is also expressed at low levels in several clusters of cells in the body wall region. (W) CG8483 is expressed along the DV margin and in several clusters of cells in the hinge and body wall regions. (X) Dorsocross 1 (Doc1) is expressed in four patches of cells including a group of cells in the body wall (arrowhead). (Y) wengen/CG6531 is expressed in the wing pouch with stronger expression in future veins and weakly in the body wall region (cells out of plane of focus).