Fig. 3. Cell shape is mildly perturbed in DMYPT mutant embryos. (A) Phosphotyrosine immunostaining of the ectoderm and amnioserosa of wild-type (wt) and DMYPT mutant (DMYPT^2-199) embryos at an early stage of dorsal closure (top panels) and midway through dorsal closure (bottom panels). The cell shape, organization and polarity of the mutants is comparable to wild type. (B) Immunostaining for the ectodermal marker, fasciclin III, reveals orderly cell elongation at the leading edge of wild-type embryonic epidermis (arrowhead). DMYPT mutant embryos (DMYPT^2-199, DMYPT⁰³⁸⁰²) show occasional disruption of cell shape and elongation toward the end of dorsal closure (lower left panel, arrowheads). All panels are lateral views of embryos with anterior to the left.