Fig. 5. DMYPT is required for ring canal growth. Filamentous actin staining was used to reveal ring canals of egg chambers. (A,B) Stage 8 egg chambers from (A) wild type (OreR) and (B) DMYPT⁰³⁸⁰² GLCs. Notice that the ring canals (arrows) in wild-type egg chamber are much larger than those in DMYPT⁰³⁸⁰² GLCs. The insets are higher magnification views of regions of stage 10 egg chambers. (C) Egg chambers of wild type (stages 3, 5, 7 and 9). (D) Egg chambers of DMYPT⁰³⁸⁰² GLCs (stages 3, 8 and 10). Note that in wild type, the ring canals grow as the egg chambers progress through oogenesis. This does not occur in DMYPT⁰³⁸⁰² GLCs.