Fig. 3. Effect of mitochondrial inhibitors on Ca²⁺ levels and Ca²⁺ signals in ascidian eggs (Phallusia). Unfertilized eggs are injected with CG/TR only (A) or with CG/TR + 5 µM cIP₃ (B,C) are perfused with CN^- (C) and FCCP (B) with or without oligomycin. Ca²⁺ transients are induced every 3 minutes by a UV flash photoreleasing Ins(1,4,5)P₃ (black arrowheads). Rates of [Ca²⁺]_c increase induced by the three mitochondrial inhibitors on the egg are compared in the bar graph shown in D (n=17). (E-I) The actions of CN^- (F,I) and FCCP (G,H) on the mitochondria in the presence (H,I) or absence (F,G) of oligomycin, compared with the untreated egg (E).