Fig. 4. VP16En represses the expression of en and hh, two positive targets of En. Wild-type embryos are shown on the left and paired-Gal4 UAS-VP16En embryos on the right. (A,B) Expression of hh in wild type (A) and in an embryo expressing VP16En (B). (C,D) Expression of en in a wild-type embryo (C) and in an embryo expressing VP16En (D). A specific probe that does not recognize VP16En was used to detect en mRNA. In B and D, expression of the target gene (hh and en, in purple) is clearly repressed in the cells that express VP16En (labelled in ochre).