Fig. 5. Expression of slp is repressed by En. (A) Expression of slp in a wild-type st11 embryo. (B) Expression of slp in an embryo that expresses both ectopic en and lacZ under the control of paired-Gal4 [prdG4(Z) UAS-En]. slp transcripts (black) disappear where En is ectopically expressed (here the domain of ectopic expression is recognized with a lacZ probe in red). (C) Abdominal region (A5-A8) of a wild-type embryo showing the expression of slp at high magnification. (D) Similar view of an st11 embryo expressing VP16En under the control of paired-Gal4. Notice the broadening of the slp stripes in odd-numbered segments, where paired-Gal4 is active.