Fig. 2. Cytoskeletal organization in expanding epidermal pavement cells of wild-type, brk2 and brk3 maize leaves. (A) In wild-type cells, cortical microtubules form bands focused at lobe sinuses; these bands are also observed in brk2 (B) and brk3 (C) cells (arrows). In the wild type, FITC-phalloidin staining of fixed cells (D) and GFP-talin expression in living cells (G) reveal enrichments of cortical F-actin localized at lobe tips (arrowheads). Such cortical F-actin patches are not observed in brk2 and brk3 cells fixed and stained with FITC-phalloidin (E and F, respectively) or expressing GFP-talin (H and I, respectively). Scale bars: 13 µm.