Fig. 4. Sema5A inhibition of post-optic disc retinal axons. (A) Diagram of the retina-optic nerve preparation. (B) Retinal axons and growth cones extending from the cut optic nerve of a retina-optic nerve preparation (boxed region in A), stained with TexasRed-Phalloidin. (C) The response rate of growth cones from retina-optic nerve preparations and standard retinal explants to oligomerized ECD-Fc. Growth cone responses were observed with time-lapse microscopy. Scale bar, (B) 50 µm.