Fig. 3. Kinetics of UFO expression following an 8-hour pulse of ethanol induction in ufo-2 35S::UFO_ind apices. (A,B) The expression levels of UFO (A) and the control APT (B) were analysed by RT-PCR at the indicated times following the beginning of an 8h-vapour induction in ufo-2 35S::UFO_ind apices. (C) The expression level of UFO was quantified as a ratio of the level of APT and was given the arbitrary value 1 at time 0h. Bars represented SE of 6 replicates. (D) Equal amounts of RT-PCR products obtained after amplification of the UFO transcripts from apices of wild-type (WT), ufo-2, or ufo-2 35S::UFO_ind plants following an 8-hour ethanol induction were digested by AflIII to discriminate between wild-type and mutated transcripts.