Fig. 2. Polyclonal origin of A101⁺ cells. hsp-flp/+; A101/Act>CD2>Gal4, UAS-GFP ovarioles stained with anti-ß-galactosidase (red/A101, A,B,D) or anti-Fas III antibodies (red, C), GFP (green, A-D), and DAPI (white, A-D). In A-D, yellow marks the superimposition of A101 or Fas III staining and GFP staining. (a1-d1) Magnifications of the boxed regions in A-D showing A101 (a1,b1,d1, red) or Fas III (c1, red) staining and GFP staining (green). (a2-d2) Magnifications of the boxed regions in A-D showing A101 (a2,b2,d2) or Fas III (c2) staining. As shown in c2, Fas III accumulates most strongly at the contact surface between the two polar cells of a given pair in late egg chambers. F III denotes Fas III.