Fig. 4. Polar cell differentiation is delayed in fused mutant females. (A,B) fu^JB3; A101/+ ovarioles double stained with DAPI (A,B) and anti-ß-galactosidase antibodies (A',B'). Higher magnification of two groups of three A101⁺ cells (inset A') and two groups of 5 and 6 A101⁺ cells (left and right, respectively, inset B') are shown. The A101 enhancer-trap line was used to visualize polar cells since introduction of this insertion in a fu mutant context does not modify fu ovarian phenotypes. (C) Percentage of fu^JB3/FM6 (blue) and fu^JB3 (pink) stage 1 to 5 egg chambers containing 0, 1, 2, 3 or 4 and more polar cells (n23 for each stage). Note that reported data are of anterior polar cells.