Fig. 5. Egg chambers with extra polar cells exhibit late defects in anterior somatic cell morphogenesis. (A,B) Control (A) and hsp-flp/+; +/UAS-p35; Act>CD2>Gal4, UAS-GFP/+ (B) egg chambers double-stained with phalloidin (green) and anti-Fas III antibodies (red). Magnification of the basal follicular epithelium (insets, bottom-left) and the anterior polar cells (insets, top-right) are shown. The bright fringe-like structure visible in B (bottom-right) are the remnants of the external sheath. (C-F) Control (C-C'',E,E'), and hsp-flp/+; +/UAS-p35; Act>CD2>Gal4, UAS-GFP/+ (D-D'',F,F') ovarioles triple-stained with DAPI (C,D,E,F), phalloidin (C',C'',D',D''E',F', green) and anti-Fas III (C',C'',D',D'',E',F', red). Magnification of stage 9 anterior polar cells and the adjacent follicular epithelium (insets, C'',D''), and of stage 10 border cells and polar cells (insets, E,F and E',F', respectively) are shown. Four and 5 polar cells are found anteriorly in D'' and F', respectively.