Fig. 5. Leydig cells in Dax1-deficient are restricted to the ventral surface. In situ hybridization for Scc reveals that while Leydig cells are present on the ventral surface of Dax1-deficient gonads (B,D) similar to wild-type males (A,C), but decreased in number and organization. Lateral examination (D) reveals that Leydig cells do not extend throughout the full ventral to dorsal thickness of the Dax1-deficient testis (arrow). Immunohistochemistry for Sf1 demonstrates that Sf1-positive Leydig cells are restricted to the coelomic surface in the Dax1-deficient testis (above the broken line, F) compared with the wild-type testis (E). Arrows identify the columns of Leydig cells in wild-type testis (E).