Development -- Castagnetti and Ephrussi 130 (5): 835 Figure IG2

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Fig. 2. osk mRNA localization is only mildly affected in orb^mel mutants. Whole-mount in situ hybridization (upper panel) was performed on ovaries from wild-type and orb^mel homozygous flies, using a DIG-labeled osk probe. The result obtained by RNA in situ hybridization was confirmed by immunostaining with ${alpha}$ -Stau antibody (lower panel).