Fig. 4. The transcriptome is stable up to the four-cell stage and changes dramatically thereafter. (A) The x-axis shows ten pairs of time points analyzed by paired-timepoint ANOVA. The y-axis shows the number of RD genes with P<0.01. The number of genes making the cut-off is also split according to whether the change in abundance is positive (Up) or negative (Down). (B) A scatter plot of the 8890 RD genes showing changes in abundance that occur between the PC6 and PC32 time points (one-cell and early four-cell stages, respectively). The max of the two mean transcript abundances is plotted on the x-axis on a log₁₀ scale. Fold-change (PC32/PC6) is plotted on the y-axis on a log₂ scale. The two lines crossing the y-axis at ±2 mark twofold changes. Each point is color coded according to whether or not the observed difference is statistically significant (P<0.01) according to a paired-timepoint ANOVA. The number of genes that are considered to be significantly different is 217, 38 of which show an increase and 179 show a decrease. (C) A scatter plot of the 8890 RD genes reflecting changes in transcript abundance that occur between the PC32 and 83 minute time points (early four-cell and 40-cell stages, respectively). The plot is otherwise identical to B. Of the 3773 genes that are considered significantly different, 1911 show an increase and 1862 show a decrease.