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Fig. 5. Markers of early placode induction are absent in hsy. (A-F) In situ hybridizations with three- to five-somite stage embryos: dorsal views, anterior towards the left. (A,C,E) wild-type and (B,D,F) hsy embryos. (A,B) pax8 expression, (C,D) pax2a expression and (E,F) dlx3b expression. (G) TaqI genotyping for dlx3b in situ. Migration position for hsy mutant and wild-type fragments are indicated at the left of the gel. mhb, midbrain-hindbrain boundary; ot, otic primordia; pn, pronephros.