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Fig. 7. Expression of markers for branchial arches and late-otic development is affected in hsy. RNA in situ hybridization with (A,C,E) wild-type and (B,D,F,G) hsy mutant embryos. (A,B) dlx4b expression, 16-somite stage. (C,D) dlx3b expression, 24 hpf. (E-G) dlx5a expression, 24 hpf. Note the loss of visceral arch expression in G. Forebrain and olfactory placode expression is unaffected in hsy. Lateral views, anterior towards the left in all panels. ot, otic placode; ov, otic vesicle; va, visceral arches.