Fig. 3. A comparison of overall genome expression patterns among pleiotropic cop/det/fus mutations and high light intensity. (A) The number of differentially expressed genes (2-fold regulation) in representative cop/det/fus mutations or high-intensity light. Six-day-old dark-grown (hatched bars) and white light-grown (white bars) mutant seedlings were examined. Lane 1, cop1-8 vs. WT; lane 2, det1-6 vs. WT; lane 3, fus6-1 vs. WT; lane 4, cop10-1 vs. WT; lane 5, high light vs. dark (hatched bar) or normal light (white bar). (B) The number of oppositely regulated and newly regulated genes by the pleiotropic cop/det/fus mutations in darkness and high light intensity. Lane 1, cop1-8/D vs. WT/D; lane 2, det1-6/D vs. WT/D; lane 3, fus6-1/D vs. WT/D; lane 4, cop10-1/D vs. WT/D; lane 5, WT/HWL vs. WT/D. Only those genes that exhibited twofold or more differential expression in each sample pair and showed oppositely regulation by a cop/det/fus mutation and normal light were selected as oppositely regulated genes. Those genes that exhibited twofold or more differential expression in cop/det/fus mutants in darkness but did not change (ratio between 0.8 and 1.2) in a normal light sample pair were selected as newly regulated genes. (C) The number of oppositely regulated genes and newly regulated genes by the cop/det/fus mutations in normal and high light intensity. Lane 1, cop1-8/WL vs. WT/WL; lane 2, det1-6/WL vs. WT/WL; lane 3, fus6-1/WL vs. WT/WL; lane 4, cop10-1/WL vs. WT/WL; lane 5, WT/HWL vs. WT/WL. Only those genes that exhibited twofold or more differential expression in each sample pair and showed opposite regulation between light-grown cop/det/fus mutants and light-grown wild type were selected as oppositely regulated genes. Those genes that exhibited twofold or more differential expression in light-grown cop/det/fus mutants as compared to wild type but did not change (ratio between 0.8 and 1.2) in normal light versus dark-grown seedlings were selected as newly regulated genes.