Fig. 8. Comparison of genome expression profiles of COP4 and DET2 with the pleiotropic cop/det/fus mutants and light regulation. (A) Hierarchical clustering display of expression profiles for cop4, wild type and four representative pleiotropic cop/det/fus mutants. The six lanes in the cluster analysis are: 1, cop4/D vs. WT/D D; 2, det1-1/D vs. WT/D; 3, WT/WL vs. WT/D; 4, cop1-1/D vs. WT/D; 5, cop9-1/D vs. WT/D; 6, cop10-1/D vs. WT/D. Those genes that exhibited twofold or more differential expression in cop4 mutant and corresponding genes in other pairs were included for comparison. A total of 496 genes were included in the cluster analysis. (B) Hierarchical clustering display of expression profiles for det2, wild type and four representative pleiotropic cop/det/fus mutants. The six lanes are: 1, det2/D vs. WT/D; 2, WT/WL vs. WT/D; 3, cop9-1/D vs. WT/D; 4, det1-1/D vs. WT/D; 5, cop1-1/D vs. WT/D; 6, cop10-1/D vs. WT/D. Only those genes that exhibited twofold or more differential expression in the det2 mutant were included for comparison. A total of 253 genes were included in the cluster. (C,D). The percentage of up- (C) or down-regulated (D) genes (exhibiting twofold or more differential expression) in the cop4 mutant that are similarly or oppositely regulated by light or each of the 4 representative pleiotropic cop/det/fus mutations. The six sample pairs selected (bars 1-6) are the same as in the lanes in A. (E,F) The percentage of up- (E) or down-regulated (F) genes (exhibiting a twofold or more differential expression) in the det2 mutant that were similarly or oppositely regulated by light or each of the 4 representative pleiotropic cop/det/fus mutations. The six bars in the diagram correspond to the lanes in B.