Fig. 7. Summary of vulva rescue experiments. (A) Organization of the basic Cel-lin-39 construct pKG11 (Garndien and Sommer, 2001). The construct contains more than 10 kb upstream region, all exons and introns and the 3'-UTR of Cel-lin-39. As in the case of Cel-mab-5, the endogenous gene has been silenced by a frame-shift mutation and cDNAs can be inserted into a unique SmaI (S) site. (B) The four constructs with Cel-lin-39, Cel-mab-5 and chimeric cDNAs inserted into pKG11. See text for details. All transgenic animals were analyzed in the strain MT4498 carrying the null allele Cel-lin-39(n1880), which is completely egg-laying defective. At least three transgenic lines were tested for each construct.