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Fig. 2. mRNA expression of Irx4 and the Slit/Robo family genes in chicken retina. In situ hybridization with various probes on retinal sections: (A) Irx4 probe, E7 retina; (B) Irx4 probe, E12 retina; (C) Slit1 probe, E4.5 retina; (D) Slit1 probe, E6 retina; (E) Robo1 probe, E9 retina. Note that Irx4, Slit1 and Robo1 are all expressed in subsets of cells in GCL. (F) Two-color in situ hybridization on E7 flat-mount retina, with Slit1 (fluorescein-labeled, shown as red) and Irx4 probes (DIG-labeled, shown as purple). Note that Slit1 and Irx4 are mostly localized in two distinct cell populations in GCL. Scale bars: 100 µm.