Fig. 5. Parapineal formation precedes asymmetric expression of lov. Expression of otx5 (blue) and lov (red) in wild-type sibling embryos at the indicated times post-fertilization. The otx5-expressing parapineal (blue arrowhead) can be distinguished at 28 h. Expression of lov (red arrowhead) is first detected at 40 h and is always found at higher levels on the left side of the brain. Scale bar: 50 µm.