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Fig. 1. Isolation of larger body size mutants. (A) Body volumes of 4-day old adults. The numbers of examined animals are as follows: N2 476, ks16 86, ks60 211, ks61 168, ks62 80, n477 53, n478 17, Ex[myo-3::gfp] 42, ks61/+;Ex[myo-3::gfp] 41, ks60/+ 19, him-5 (e1490) male 10, ks61;him-5 male 15, lon-1(e185) 10, lon-2(e678) 43, lon-3(e2175) 43, daf-4(m63) 13, daf-16(mgDf50) 32, daf-16(mgDf50);ks61 37 (results with two lines), daf-2 (e1370) 67, daf-2 (e1370);ks61 157 (results with three lines), daf-3 (mgDf90) 46, ks61;daf-3 (results with three lines) 74, sma-6 (e1482) 29, sma-6;ks61 52, dbl-1 (nk3) 86 and ks61;dbl-1 32. Error bars indicate standard deviation (s.d.) (B) DIC microphotographs of a 4-day old adult of N2 (top) and a larger body size mutant (ks60, bottom). The pictures were taken in 5-8 parts per animal using an AxioCam CCD camera mounted on Zeiss Axiophot 2 with a 20x/0.5 lens and assembled. Scale bar: 200 µm.