Fig. 5. Characterization of endogenous EGL-4 proteins. (A) Extracts of wild-type N2 and indicated mutants (10 µg protein each) were separated in a 7.5% polyacrylamide-SDS gel, subjected to western blotting and probed with purified anti-EGL-4 antibodies. (B) Protein kinase activities in extracts from egl-4 (ks16) (lane 1) or N2 (lanes 2-6) were assayed in the absence (lane 2) and presence of 0.1 µM (lane 3), 1 µM (lane 4) or 10 µM (lanes 1, 5, 6) cGMP. For lane 6, the antibodies were preincubated with the antigen GST-EGL-4.