Fig. 5. Effects of soluble neuropilin/Fc reagents on repulsion by the notochord of stage 26-27 DRG and stage 36 SCG axons in collagen gel co-cultures. (A-C) Stage 26-27 DRG explants cultured with (A) 10 µg/ml neuropilin 1/Fc (NP1), (B) 10 µg/ml neuropilin 2/Fc (NP2) and (C) 5 µg/ml each of neuropilin 1 and 2/Fc (NP1/2); (n=27, 20 and 11, respectively). (D-F) As for A-C, but with stage 36 SCG explants (n=24, 16 and 10, respectively). (G) Histogram showing the mean repulsion score±s.e.m. (n=24 and 27 for DRG and SCG explants, respectively, in the absence of neuropilin/Fc reagents. The neuropilin/Fc reagent dose in µg/ml is indicated in brackets). Asterisks indicate a significant reduction in repulsion compared to controls. Scale bar: 100 µm.