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Fig. 8. Axon repulsion of other neuronal populations by the notochord. (A) Axons from stage 26-29 retinal explants are repelled by the notochord (n=52), but axons from stage 36 olfactory bulb explants are not (B) (n=20). (A,B) Co-cultures viewed by phase-contrast microscopy. Scale bar: ~100 µm. (C) Histogram showing the mean repulsion score±s.e.m. Soluble neuropilins do not reduce the repulsion of retinal axons by the notochord; n=11 for both neuropilin 1/Fc (NP1) and neuropilin 2/Fc (NP2), respectively. Asterisk indicates no significant repulsion.