Fig. 5. Refining the position of silencer sequences. Each panel shows a germarium recovered from females expressing green fluorescent protein (GFP) reporter from transgenes driven by the named promoter fragments. Arrows indicate the position of the terminal filament base, whereas asterisks mark GSCs when these cells are GFP-negative in (A) –799/+55-GFP; (B) –799/+34-GFP; (C) –799/+55(+27-+37)-GFP; (D) –799/–20(+27-+37)-GFP; (E) –799/+150(+37-+39)GFP; (F) –799/–20(+25-+45)-GFP. (G) An example of an ovariole with germline clones from brk[M68] P{FRT}18A/P{Ubi-GFP} P{FRT}18A flies that were heat-shocked 14 days prior to ovary dissection. The arrow points to the fusome in a GFP-negative GSC; several cysts with GFP-negative cystocytes are present.