Fig. 7. Silencer activity is position dependent. A reporter construct (A) was constructed to place the silencer element upstream of a bam promoter that contained only the positively acting elements. GFP expression from the transgene included GSCs. (B) GFP expression from a construct that replaces the putative basal bam promoter element with a P-element minimal promoter element. (C) GFP expressed in the native bam pattern from a promoter fragment that includes the region from –402 to +133. (D,E) GFP expression patterns of promoters derived from the same fragment as in C but carrying deletions of –86 to –61 (D) and –68 to –61 (E) are shown. Arrows point to position of terminal filament base in A and B; asterisk marks GSC in C.