Fig. 3. roadblock (robl) mutants affect axonal transport and neuroblast proliferation. (A,B) Neuroblast clones homozygous for robl^MB examined in wandering third instar larva (A) and in adult (B). Red staining in B represents FasII immunoreactivity, which labels strongly the last-born class of /ß neurons. The dorsal axons in robl^MB neuroblast clones (green) are therefore ' axons, as they are not stained with FasII. (C) Wild-type mushroom body neuroblast clone double labeled with FasII (red). (D) A robl^B neuroblast clone examined in wandering third instar larva. (E) A robl^Z neuroblast clone examined in adult. (F,G) Axons of neurons in adult medial lobe from single-cell clones of wild type (F) or robl^Z (G). Long arrows in all images indicate accumulations of intense mCD8-GFP staining most probably because of accumulation of cargoes along the axons. Short oblique arrow in E indicates the cell body region of this neuroblast clone.