Fig. 2. Amino acid sequence of CHE-1. (A) Nucleotide sequence of the che-1 cDNA and amino acid sequence of the predicted product (CHE-1). Splice junctions were determined by comparison with the genomic sequences and are marked with vertical lines. The putative termination codon is marked with double underlines and an asterisk. The zinc-finger region is underlined. The invariant cysteine and histidine residues of C2H2-type zinc-finger motifs are boxed. Mutation sites are indicated by diamonds with resultant nucleotide and amino acid substitutions. (B) Genomic organization of the che-1 gene. Sequences encoding the zinc fingers are shaded. Seven che-1 mutations are indicated along with their respective nucleotide and predicted amino acid alterations. (C) Alignment of the zinc fingers of CHE-1 with the zinc fingers of the Drosophila protein GLASS (Moses et al., 1989). Identical residues are shaded gray. Conserved cysteine and histidine residues are highlighted. Mutation sites in CHE-1 are indicated by a black circle (nonsense) or white circles (mis-sense).