Fig. 3. Expression pattern of che-1 and rescue of chemotaxis defect by che-1 constructs. (A) A che-1 genomic clone and gfp fusion constructs. Transformed lines with extrachromosomal arrays of each of these constructs in the che-1(p679) mutant background were examined for rescue of chemotaxis as in Fig. 1. GFP expression pattern was examined in wild-type background. (B,C) Overlap of DIC and fluorescent images showing expression of pche-1::gfpBglII (B) or pche-1::gfpC (C) in wild-type L2 stage animals. Scale bars: 10 µm. (D-F) Tracks of animals showing chemotaxis behaviors on an agar plate with a concentration gradient of NaCl: (D) wild type, (E) che-1(p679), (F) che-1(p679); Ex[pche-1-HindIII (che-1(+)), plin44p-gfp]. Although the che-1 mutant failed to respond to the concentration gradient, the wild-type and the rescued animals migrated to the concentration peak at the center and remained there.