Fig. 4. dap oocytes accumulate Orb protein and progress to pachytene. (A) A dap cyst (arrowhead) in region 2b of the germarium accumulates Orb protein (green) in a manner indistinguishable from an adjacent wild-type cyst (arrow). In both (B) wild type and (C) dap clones, in region 2b of the germarium the two pro-oocytes contain thread-like C(3)G staining (green) indicative of cells in pachytene. In both panels, the other 14 cells in the cyst that are negative for C(3)G are not shown. In B,C the cell with the stronger C(3)G staining is the true oocyte (arrow). In A,C dap clones were identified by the absence of ß-gal staining (red). Scale bar: 5 µm.