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Fig. 3. GFP:MEX-5 and PIE-1:GFP dynamics in wild-type embryos (Movies 5 and 6). (A) Pronuclear formation. (B-D) Pronuclear migration. (D) Pseudocleavage. (F) Pronuclear meeting. (G) Mitosis. (H) Two-cell stage. Note that PIE-1:GFP accumulates in the male pronucleus during pronuclear migration, on the posterior centrosome during mitosis, and in the P1 nucleus in the two-cell stage. PIE-1:GFP and GFP:MEX-5 also accumulate on the cytoplasmic P granules (best seen in the movies).