Fig. 1. DM2, DM3 and VA1lm glomeruli are mis-shapen and ectopically localized in dock and Pak mutants. Confocal micrographs of adult ALs stained with the nc82 (red) and anti-GFP (green) antibodies, revealing the neuropil structure. (A) Wild-type ALs showing distinct anatomical subdivisions: the glomeruli. (B) A pair of dock^P1/dock^P1 ALs and (C) a pair of Pak⁶/Pak¹¹ ALs showing an amorphous neuropil structure. (D-F) DM2, (G-I) DM3 and (J-L) VA1lm glomeruli were visualized by expressing UAS-nsyb::GFP with Or22a-Gal4, Or47a-Gal4 and Or47b-Gal4, respectively. In the wild type (D,G,J), DM2, DM3 and VA1lm have characteristic shapes and are located in invariant positions in the AL. In dock^P1/dock^P1 (E,H,K), and Pak⁶/Pak¹¹ (F,I,L) mutants, DM2 and DM3 glomerular structures are smaller and scattered randomly in the AL. While VA1lm is located approximately in the right position, it is severely misshapen and frequently engulfs the adjacent VA1d glomerulus. Scale bar: 20 µm.