Fig. 3. Loss of forebrain in Cnbp mutants. (A,B,K,L) Bf1 mRNA, a marker for the telencephalon was entirely absent in E8.5 (B) and E9.5 (L) Cnbp^-/- embryos (n=5). (C-F) Hesx1 and Six3 markers, used here to label the diencephalon, were entirely absent in E8.0 Cnbp^-/- embryos (D,F) when compared with wild-type littermates (C,E). (G,H) En1, a marker for midbrain and anterior hindbrain, is normally expressed in E8.5 wild-type and Cnbp^-/- mutant embryos. (I,J) Expression of Krox20, a marker for rhombomeres 3 and 5, is observed in E9.0 wild-type and Cnbp^-/- mutant embryos. (M,N) Mox1 expression is normal in paraxial mesoderm cells in E9.5 Cnbp^-/- mutant when compared with their wild-type littermates. (O,P) Brachyury (T) expression is detected in the notochord and posterior mesoderm cells of mutant embryos at E9.5. T expression in tail was similar in homozygous mutants and their wild-type littermates.