Fig. 4. Molecular analyses of the origins of the developmental defects in Cnbp^-/- mutants by whole-mount in situ hybridization analysis of marker genes. Lateral views of embryos are shown with anterior (A) to the left and posterior (P) to the right. (A,B) Whole-mount in situ hybridization with Hex probe at E6.0. Hex was expressed normally in the distal end of the epiblast of Cnbp^-/- mutant when compared with wild-type littermates. (C,D) Hex was expressed in the displaced AVE of E6.5 wild-type embryos but was retained near the distal end of the epiblast in the mutants (arrow). (E,F) Lim1 is expressed in the AVE and the primitive streak of the E6.5 wild-type embryo; however, transcripts are more towards the distal end of the AVE in Cnbp^-/-. (G,H) Hex is expressed in the anterior definitive endoderm (ADE) and AVE of E7.25 wild-type embryos but is not detectable in E7.25 mutant embryos. (I,J) Cer1 expression was undetectable in the ADE and AVE in E7.25 mutant embryos. (K,L) Hex is expressed in the ADE and ANE of E7.5 wild-type embryos but is not detectable in that of E7.5 mutant embryos. (M,N) Cer1 expression was undetectable in the ADE and ANE in E7.5 mutant embryos. (O,P) Expression of Otx2 in the ANE was not observed in mutant embryos.