Fig. 6. Effect of time-dependant activation of Notch in Dll1^lacZ/lacZ mutant spheres on the production of OPCs (B, I-III) and on oligodendrocytes (B, IV-VI). (A) Experimental protocol: 24 hours (left panel) or three-day-old (right panel) Dll1^lacZ/lacZ mutant spheres were incubated in the presence of J1^EC for various time intervals. (B) Spheres were immunostained with anti-MAP2 (red) and anti-PDGFR (green) for OPCs production (I-III); anti-04 (green) for oligodendrocytes production (IV-VI). (C) Quantitative estimations of neurons and OPCs (from I-III) was as described in experimental procedures. Data are representative of three independent experiments. Scale bar: 50 µm.