Fig. 5. Rac1V12 prevents newly synthesized E-Cadherin from accumulating at the apical cell membrane. (A-D) Embryos with the genotypes of btl-Gal4, UAS-E-Cadherin-GFP / + (control: A,C) and btl-Gal4, UAS-E-Cadherin-GFP / +; UAS-Rac1V12 / + (Rac1V12: B,D) at two different stages were labeled with anti-GFP (green) to reveal E-Cadherin-GFP and with DCAD2 (purple) to reveal both E-Cadherin-GFP and endogenous E-Cadherin. Note that in the control (A,C) and in a stage 11 Rac1V12 embryo (B), anti-GFP and DCAD2 signals overlap, but in D the signals are distributed differently.