Fig. 7. A tollo-Gal4 transgene drives lacZ expression (from
UAS-lacZ) in the same cells that express Tollo mRNA. (A,C)
lacZ activity visualized in tollo-Gal4; UAS-lacZ
embryos. (B,D) Tollo mRNA detected in OreR embryos by in situ hybridization.
In stage 12, germ band-extended embryos (A,B), lacZ activity (A) is
detected in segmentally repeated bands of ectodermal cells that mirror Tollo
mRNA (B). In the peripheral ectoderm at stage 13 (C,D) lacZ activity
(C) is detected in domains of ectodermal cells. Segments T3 A2 are
shown in C,D, anterior towards the left and dorsal towards the top of the
panel. Scale bar: 350 µm in A,B; 60 µm in C,D.