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Fig. 5. Fgf8 and Hnf3b expression patterns are rescued in the tail bud and hindgut of A1-/-{gamma}-/- mutants. Fgf8 is normally (A) expressed in the midbrain-hindbrain junction (white asterisk), maxillary (mx) and mandibular (md) components of the first branchial arch, otic vesicle (ov), commissural plate (cp), heart (ht) and associated vasculature, hindgut (hg), and tail bud (tb) nascent mesoderm. (D) Hnf3b expression is specific of the midbrain (mb), notochord (nc), foregut (fg) and hindgut (hg). In the A1-/- mutant (B), Fgf8 hindgut labeling (hg*) stops at the level of the posterior neuropore (PNP, arrowhead) and expression is down-regulated in the nascent mesoderm of the malformed, twisted tail bud tissues (tb*; see inset). Although Hnf3b notochord labeling persists in the everted tail bud tissues of the A1-/- mutant (E, inset), hindgut labeling ends at the level of the PNP (arrowhead). A1-/-{gamma}-/- double-mutants show normal Fgf8 and Hnf3b patterns of expression (C and F, respectively). aer, apical ectodermal ridge.