Fig. 2. Inhibition of MAP-kinase activity does not inhibit Ca²⁺ oscillations. Treatment with UO 126 inhibited MAP-kinase activity in MII eggs and maintained low levels of MAP kinase up until Pn formation when it would normally decline. Kinase assays as for Fig. 1. Ca²⁺ oscillations in UO 126-treated eggs (n=38) were similar to controls (B). The cessation of oscillations correlated tightly with Pn formation (C) (compare with Fig. 1C, see Table 1).