Fig. 2. mRNA and protein expression in the BDNF^NT3/NT3 mice at P14. (A-D) BDNF and NT3 in situ hybridisation of brain sections. Scale bars:100 µm in A-D. Note that NT3 mRNA expression changes in BDNF^NT3/+ (C) and BDNF^NT3/NT3 (D) mice towards the same expression pattern as BDNF in wild-type animals (A). (E-F) Analyses of protein levels in hippocampus using BDNF and NT3 ELISA. (E) BDNF protein levels decrease in BDNF^NT3/+ mice and are completely absent in BDNF^NT3/NT3 animals. (F) NT3 protein levels increase in BDNF^NT3/+ and BDNF^NT3/NT3 mice to reach a similar level as BDNF protein in wild-type animals.