Fig. 4. Suppression of CT neuron generation by MNR2, HB9 and Lim3. (A) Widespread ectopic expression of MNR2 protein on the right side of spinal cord after MNR2 electroporation. (B-D) Loss of dorsomedially located Isl1⁺ motor neurons (B), and absence of expression of BMP5 (C) and ephrinA5 (D) after unilateral MNR2 electroporation. (E) Widespread ectopic expression of HB9 protein on the right side of spinal cord after HB9 electroporation. (F-H) Loss of dorsomedially located Isl1⁺ motor neurons (F) and absence of expression of BMP5 (G) and ephrinA5 (H) after unilateral Hb9 electroporation. (I) Widespread ectopic expression of Lim3 protein on the right side of spinal cord after Lim3 electroporation. (J-K) Loss of dorsomedially located Isl1⁺ motor neurons (J) and absence of BMP5 expression (K), after unilateral Lim3 electroporation. (L) Ectopic dorsal Chx10⁺ (V2) interneurons induced by Lim3 electroporation. Brackets indicate area of expression on the untreated side. Images are representative of 10-30 electoporated embryos.