Fig. 5. Hierarchical interactions between MNR2, HB9 and Lim3 in the suppression of CT neuron generation. (A-E) Electroporation of MNR2 within the normal domain of motor neuron generation at thoracic levels (A) represses BMP5 expression (B), but does not result in maintained Lim3 (C,D) or HB9 (E) expression in motor neurons. (F-J) Electroporation of Lim3 within the normal domain of motor neuron generation at thoracic levels (F) represses BMP5 expression (G), and results in maintained MNR2 (H) and HB9 (I,J) expression in postmitotic motor neurons. Images are representative of 20 electroporated embryos. Arrows in C-E,H-J indicate the approximate position of newly generated CT neurons, prior to their dorsal migration.