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Fig. 8. Inductive activity of a MNR2-E1a C-terminal domain fusion protein. (A) Position of PxxL motifs in MNR2 and related Mnx class HD proteins. (B) Diagram of MNR2HD-E1a C-terminal fusion proteins. (C) Widespread ectopic expression of MNR2 HD-E1a C-terminal fusion protein. (D) Lim3 inductive activity of MNR2 HD-E1a C-terminal fusion protein in vivo. (E) Widespread expression of the mutated MNR2 HD-E1a C-terminal fusion protein. (F) Lack of Lim3 inductive activity of MNR2 HD-E1a C-terminal fusion protein mutated in its three C-terminal residues. (G) Widespread expression of MNR2 HD-E1a C-terminal fusion protein in spinal cord at stage 29 after in ovo electroporation. (H) Repression of CT neuron generation (arrow) by MNR2 HD-E1a C-terminal fusion protein. The MNR2 HD fused to a VP-16 activation domain was also inactive in inducing Lim3 expression (data not shown). Images are representative of over 20 electroporated embryos per construct.