Fig. 8. Somatic defects in mat-1 mutants. DIC images of tails from wild-type (A) or mat-1 (B-D) young adult males that had been shifted from 15°C to 25°C as L1 larvae. ax161 tails (C) are essentially normal, ax212 tails (B) exhibit moderate defects including missing or fused rays, and the severely reduced ax144 tails (D) typically lack rays altogether. Posterior regions of wild-type (E,F) and ax144 (G,H) males that were shifted to 25°C as L1 larvae and processed for immunofluorescence as L4 larvae for DAPI (E,G) and p-H3 (F,H). In comparison to the wild-type controls (E,F), ax144 animals exhibit reduced cell proliferation as assessed by DAPI-staining (G) and increased numbers of M-phase nuclei as assessed by anti-p-H3 immunofluorescence (H). (I) Wild-type vulva (white arrowhead). (J) Everted vulva (white arrowhead). (K) Quantitation of excess p-H3-staining somatic nuclei within the posterior of mat-1 L4 males (30-36 hours after the L1 upshift).